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XNF-ATc3 affects neural convergent extension.

Annette Borchers1, Yuri Fonar, Dale Frank

  • 1Department of Genetics, Stanford University Medical School, Stanford, CA 94062, USA.

Development (Cambridge, England)
|March 31, 2006
PubMed
Summary

Nuclear Factor of Activated T-cells (NF-AT) activation inhibits Xenopus embryonic body axis elongation. Inhibiting NF-AT specifically blocks neural convergent extension, revealing its critical role in neural tube development.

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Area of Science:

  • Developmental biology
  • Molecular signaling
  • Xenopus embryogenesis

Background:

  • Convergent extension drives anteroposterior body axis elongation in Xenopus.
  • This process occurs in dorsal mesoderm and posterior neural ectoderm via similar molecular pathways.

Purpose of the Study:

  • To investigate the role of Nuclear Factor of Activated T-cells (NF-AT) in Xenopus convergent extension.
  • To determine the specific function of NF-AT in different embryonic tissues.

Main Methods:

  • Whole embryo analysis, mesodermal explants, and posterior neural explants.
  • Activation and inhibition of NF-AT signaling using dominant-negative XNF-ATc3 and calcineurin inhibitor cyclosporin.
  • Temporal and spatial approaches to analyze NF-AT function in neural tube development.

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Last Updated: Jul 12, 2026

Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons
09:28

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Published on: March 9, 2010

Neural Tube Closure in Mouse Whole Embryo Culture
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Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation
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Main Results:

  • NF-AT activation inhibits convergent extension in both dorsal mesoderm and posterior neural ectoderm.
  • Inhibition of NF-AT specifically affects neural ectoderm convergent extension in whole embryos.
  • Blocking NF-AT signaling prevents neural convergent extension, with XNF-ATc3 expressed and active in the neural tube.

Conclusions:

  • NF-AT acts as a regulator of neural convergent extension in Xenopus.
  • This study identifies XNF-ATc3 as a key molecule involved in neural tube development and body axis elongation.