Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The human cumulus--oocyte complex gene-expression profile.

Said Assou1, Tal Anahory, Véronique Pantesco

  • 1CHU Montpellier, Institut de Recherche en Biothérapie, Hôpital Saint-Eloi, and INSERM U 475, Université Montpellier, UFR de Médecine, France.

Human Reproduction (Oxford, England)
|March 31, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond amyloid and tau: synaptic and neurodegenerative biomarkers shape MCI progression.

Molecular psychiatry·2026
Same author

Comparative induced pluripotent stem cell models of young, aged, and progeroid, as a resource to study human aging trajectories.

Stem cell research·2026
Same author

The lithic assemblage from the 700,000-year-old butchery site of Kalinga (Luzon Island, Philippines): New insights into technological variability in the Early Paleolithic in Island Southeast Asia.

Journal of human evolution·2026
Same author

Human iPSC-derived airway models enable comparative analysis of SARS-CoV-2 infection in healthy and COPD bronchial epithelium.

iScience·2026
Same author

Biomarkers.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same author

Advancing diversity in access to medical studies: Evidence from a prospective cohort.

Medical teacher·2025

Researchers identified key genes involved in human oocyte maturation and cumulus cell function. This study enhances understanding of reproductive biology and offers potential biomarkers for oocyte competence and granulosa cell tumors.

Area of Science:

  • Reproductive Biology
  • Genomics
  • Oocyte Development

Background:

  • Mechanisms regulating human oocyte maturation are not fully understood.
  • Differential gene expression between immature and mature oocytes and surrounding cumulus cells was investigated.

Purpose of the Study:

  • To identify genes differentially expressed during human oocyte maturation.
  • To discover potential biomarkers for oocyte competence and granulosa cell tumors.

Main Methods:

  • Oligonucleotide microarrays were used to study genome-wide gene expression.
  • Gene expression was analyzed in pooled immature and mature oocytes and cumulus cells from IVF patients.

Main Results:

  • Oocytes up-regulated 1514 genes, including novel growth factors (e.g., TNFSF13/APRIL, FGF9) and transcription factors (e.g., OTX2, SOX15).

Related Experiment Videos

  • Specific genes like PTTG3 (securin) and AURKC (Aurora kinase) were preferentially expressed during oocyte meiosis.
  • Cumulus cells overexpressed cell-to-cell signaling genes (e.g., TNFSF11/RANKL), complement components, and semaphorins.
  • 52 genes, including CDC25A and SOCS7, showed progressive increase during oocyte maturation.

    • Conclusions:

    • Identification of differentially expressed genes advances understanding of oocyte biology.
    • Discovered genes may serve as novel biomarkers for viable and competent oocytes.
    • Genes expressed in cumulus cells could indicate potential markers for granulosa cell tumors.