Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Adenosine receptor expression and function in bladder uroepithelium.

Weiqun Yu1, Lefteris C Zacharia, Edwin K Jackson

  • 1Univ. of Pittsburgh, Renal Division, 982 Scaife Hall, 3550 Terrace St., Pittsburgh, PA 15261, USA.

American Journal of Physiology. Cell Physiology
|March 31, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Salbutamol and Formoterol Attenuate Okadaic Acid-Induced Cytotoxicity in Undifferentiated PC-12 Cells: Evidence for a β2-Adrenergic Receptor-Independent Protective Component.

Current issues in molecular biology·2026
Same author

Purinergic activity of circulating extracellular vesicles associates with disease progression in melanoma.

Oncoimmunology·2026
Same author

Rationale for the Use of 8-Aminoguanine for the Management of Cystitis.

International journal of urology : official journal of the Japanese Urological Association·2026
Same author

8-Aminoguanine protects against paclitaxel-induced neural degeneration and mechanical allodynia.

JCI insight·2026
Same author

Dysregulation of CD39/Ectonucleoside Triphosphate Diphosphohydrolase 1 Causes Urinary Bladder Dysfunction with Abnormal Smooth Muscle Contractility.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Comparative effectiveness and safety of esketamine versus injectable racemic ketamine and oral antidepressants for major depressive disorder: A population-based target trial emulation.

Journal of affective disorders·2026
Same journal

Skeletal muscle biomass as an underappreciated fate of glucose.

American journal of physiology. Cell physiology·2026
Same journal

Proteomic profile of urinary extracellular vesicles from rats with lithium-induced nephrogenic diabetes insipidus.

American journal of physiology. Cell physiology·2026
Same journal

A non-hydrolyzable candesartan cilexetil analog reveals synergistic activation as a tractable mechanism for TMEM175 modulation.

American journal of physiology. Cell physiology·2026
Same journal

Mitochondrial Calcium Transport in Amino Acid Metabolism: From nutritional responses to metabolic regulation.

American journal of physiology. Cell physiology·2026
Same journal

N-linked glycosylation regulates SNAT2 trafficking and stability in pancreatic ductal adenocarcinoma cells.

American journal of physiology. Cell physiology·2026
Same journal

Oxaloacetate Inhibition of Succinate Dehydrogenase: Mechanism and Physiological Implications.

American journal of physiology. Cell physiology·2026
See all related articles

Bladder uroepithelium releases adenosine, a molecule that regulates membrane turnover in umbrella cells via adenosine receptors. This process is crucial for maintaining the bladder

Area of Science:

  • Urology
  • Cell Biology
  • Physiology

Background:

  • The bladder uroepithelium forms a critical impermeable barrier.
  • Membrane turnover in umbrella cells maintains this barrier, but physiological regulators are poorly understood.
  • Adenosine signaling is a potential regulator of urothelial function.

Purpose of the Study:

  • To investigate the expression and function of adenosine receptors in the uroepithelium.
  • To determine the role of adenosine in regulating urothelial barrier function and membrane turnover.

Main Methods:

  • Western blot analysis to detect adenosine receptor expression.
  • Measurement of uroepithelial membrane capacitance following adenosine administration.
  • Investigation of adenosine release and its potentiation by tissue stretching.

Related Experiment Videos

  • Pharmacological manipulation using selective agonists, antagonists, and inhibitors.

    • Main Results:

    • All four adenosine receptors (A1, A2a, A2b, A3) are expressed in the uroepithelium, with distinct localization patterns.
    • Uroepithelium releases adenosine, with release significantly potentiated by tissue stretching.
    • Adenosine administration increases urothelial membrane capacitance, indicating modulation of membrane turnover, particularly via A1 receptors.
    • Adenosine-induced capacitance increases are dependent on intracellular calcium signaling pathways.

    Conclusions:

    • The uroepithelium synthesizes and releases adenosine.
    • Adenosine receptors are present and functional within the uroepithelium.
    • Adenosine signaling, particularly through A1 receptors, plays a role in regulating exocytosis and membrane turnover in umbrella cells, contributing to barrier function.