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Updated: Aug 9, 2026
09:04
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
RNAi: a novel antisense technology and its therapeutic potential
Anne Dallas1, Alexander V Vlassov
1SomaGenics, Delaware Ave, Santa Cruz, CA 95060, USA.
Summary
RNA interference (RNAi) is a potent gene knockdown technology. This review explores RNAi
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- Antisense oligonucleotides inhibit gene expression via sequence-specific RNA binding.
- Gene silencing technologies include antisense oligonucleotides, ribozymes, deoxyribozymes, and RNA interference (RNAi).
Purpose of the Study:
- To introduce the RNAi effect and compare it with existing antisense technologies.
- To discuss the therapeutic potential of RNAi, focusing on animal studies and clinical trials.
Main Methods:
- Review of existing literature on antisense technologies and RNAi.
- Comparison of different gene-silencing molecule categories.
- Analysis of recent animal studies and clinical trials for RNAi therapeutics.
Main Results:
- RNAi is a potent and widely used gene knockdown approach.
- RNAi offers therapeutic potential for various diseases including viral infections, neurodegenerative diseases, cancer, and more.
- In vivo delivery of small interfering RNAs remains a significant challenge.
Conclusions:
- RNAi represents a significant advancement in gene silencing technologies.
- RNAi holds promise for novel therapeutics, but delivery challenges need to be addressed.
- Further research is needed to overcome in vivo delivery obstacles for RNAi clinical applications.
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02:15Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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01:20Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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