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Miniaturised isotachophoresis analysis.

Lin Chen1, Jeff E Prest, Peter R Fielden

  • 1Institute for Analytical Sciences, Bunsen-Kirchhoff Str. 11, D-44139 Dortmund, Germany.

Lab on a Chip
|March 31, 2006
PubMed
Summary

Microchip isotachophoresis (ITP) offers advanced separation and sample preparation for ionic compounds and biomolecules. This review highlights microchip ITP features and applications developed since 1998.

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Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Separation Science

Background:

  • Miniaturized Total Analysis Systems (microTAS) have rapidly advanced.
  • Isotachophoresis (ITP) is well-suited for microchip format due to controlled separation parameters and high sample capacity.

Purpose of the Study:

  • To review the fundamental characteristics of microchip-based ITP.
  • To summarize applications of microchip ITP in analysis and pretreatment since 1998.

Main Methods:

  • Review of scientific literature published since 1998.
  • Focus on microchip implementations of Isotachophoresis.

Main Results:

  • Microchip ITP enables efficient separation and pretreatment of ionic compounds and biomolecules.

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  • Key features include electrolyte-controlled separation and high sample loading.

    • Conclusions:

    • Microchip ITP is a powerful technique for diverse analytical challenges.
    • Its development since 1998 has expanded its utility in various fields.