Related Experiment Video
Updated: Aug 9, 2026
Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model
Published on: February 24, 2017
[Effect of adeno-BMP7 transfection on osteogenesis of BMSCs]
Lian Zhu1, Fu-guo Chen, Wei Liu
1Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiaotong Medical University and Shanghai Tissue Engineering Center, Shanghai 200011, China.
Objective:
To study the effect of adeno-BMP7 transfection on the biology of bone marrow stromal cells (BMSCs).
Methods:
Bone marrow was obtained from the goat. The BMSCs were isolated and cultured at the second passage. Once the cells attached and formed a monolayer with 70%-80% confluency, adeno-BMP7 (M.O.I. = 100) was added to the cells. After three days, calcium node was examined with staining; cell-coral compound was replanted subcutaneously.
Results:
With adeno-BMP7 transfection, BMP7 expression was detected with Western-blot; big calcium nodes were observed with staining. New bone formation was enhanced, which was evaluated by X-ray and histological examinations.
Conclusions:
BMSCs transfected with adeno-BMP7 show much stronger osteogenic ability.
More Related Videos
10:32Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate
Published on: May 19, 2023
05:10Improved Methodology for Studying Postnatal Osteogenesis via Intramembranous Ossification in a Murine Bone Marrow Injury Model
Published on: February 7, 2025