Related Experiment Video
Updated: Aug 9, 2026

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications
Published on: January 27, 2014
Regulation of prokaryotic adenylyl cyclases by CO2
Arne Hammer1, David R W Hodgson, Martin J Cann
1School of Biological and Biomedical Sciences, Durham University, South Road, Durham DH1 3LE, UK.
Abstract:
The Slr1991 adenylyl cyclase of the model prokaroyte Synechocystis PCC 6803 was stimulated 2-fold at 20 mM total C(i) (inorganic carbon) at pH 7.5 through an increase in k(cat). A dose response demonstrated an EC50 of 52.7 mM total C(i) at pH 6.5. Slr1991 adenylyl cyclase was activated by CO2, but not by HCO3-. CO2 regulation of adenylyl cyclase was conserved in the CyaB1 adenylyl cyclase of Anabaena PCC 7120. These adenylyl cyclases represent the only identified signalling enzymes directly activated by CO2. The findings prompt an urgent reassessment of the activating carbon species for proposed HCO3--activated adenylyl cyclases.
More Related Videos
11:31Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
Published on: June 30, 2016
08:22Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and λ) Hyperspectral FRET Imaging and Analysis
Published on: October 27, 2020
Related Concept Videos
GPCRs Regulate Adenylyl Cylase Activity
Two...
Global Regulatory Systems
cAMP-dependent Protein Kinase Pathways
Positive Regulator Molecules
Positive Regulator Molecules
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...