Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The carboxyl-terminal linker is important for chemoreceptor function.

Mingshan Li1, Gerald L Hazelbauer

  • 1Department of Biochemistry, University of Missouri-Columbia, 117 Schweitzer Hall, Columbia, MO 65211, USA.

Molecular Microbiology
|April 1, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and application of an evidence-based three-dimensional, four-phase discharge preparation plan for type 2 diabetes patients.

Frontiers in public health·2026
Same author

Development and validation of a biochemical recurrence risk assessment model for prostate cancer based on TCGA database.

Translational andrology and urology·2026
Same author

Continuous MXene fibers with near-gigapascal tensile strength via radial confinement and axial stretching.

Nature communications·2026
Same author

Corrigendum to "Hydrogel-forming microneedles for rapid and efficient skin deposition of controlled release tip-implants" [Mater. Sci. Eng. C 127 (August 2021) 112226].

Biomaterials advances·2025
Same author

Correction to "Schizophrenia Treatment Based on Sustained Release of Risperidone from Poly(lactic-<i>co</i>-glycolic) Acid Implantable Microarray Patch ".

ACS applied materials & interfaces·2025
Same author

Coordinated synthesis of aromatic amino acid derived pigments in yeast via global transcriptional engineering.

Microbial cell factories·2025

Bacterial chemotaxis adaptation relies on a receptor linker region. This linker is crucial for adaptational modification, enabling effective bacterial cell movement and signaling.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Bacterial chemotaxis allows cells to move towards attractants or away from repellents.
  • Chemotaxis adaptation, crucial for sustained directional movement, is regulated by reversible methylation of chemoreceptors.
  • Methylation is catalyzed by CheR methyltransferase and demethylation by CheB methylesterase, both interacting with a pentapeptide motif on receptors.

Purpose of the Study:

  • To investigate the role of the variable linker region connecting chemoreceptors to their pentapeptide modification sites.
  • To determine how this linker region influences the efficiency of adaptational modification and overall chemotaxis in Escherichia coli.

Main Methods:

  • Creation of nested deletions within the linker region of the Tar chemoreceptor in Escherichia coli.

Related Experiment Videos

  • Assessment of chemotaxis ability in receptor mutants with varying linker lengths.
  • Analysis of receptor signaling, substrate modification rates, and enzyme binding in response to linker truncations.

    • Main Results:

    • Deletion of 20-40 residues in the linker eliminated chemotaxis, while shorter deletions reduced it.
    • Receptor signaling and substrate modification were largely intact, except in the most truncated mutants.
    • Linker truncations reduced the rate of adaptational modification, correlating directly with the loss of chemotaxis.
    • Enzyme binding studies suggested the linker facilitates pentapeptide accessibility and dual enzyme binding.

    Conclusions:

    • The linker region is essential for effective bacterial chemotaxis, primarily by facilitating adaptational modification.
    • The linker acts as a flexible element, separating the pentapeptide from the receptor body and enabling efficient enzyme-receptor interactions.
    • Structural dynamics of the linker and its associated helix are implied in the adaptation mechanism.