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Published on: December 15, 2017
From yeast ammonium transporters to Rhesus proteins, isolation and functional characterization
A-M Marini1, M Boeckstaens, B André
1Laboratoire de physiologie moléculaire de la cellule, institut de biologie et de médecine moléculaires, université libre de Bruxelles CP300, rue des professeurs-Jeener-et-Brachet, 12, 6041 Gosselies, Belgium. ammarini@dbm.ulb.ac.be
Abstract:
Saccharomyces cerevisiae possesses three ammonium transporters from the Mep/Amt family involved in ammonium acquisition and retention. We have shown that Rh proteins are structurally related to Mep/Amt proteins and that human RhAG and RhCG perform bi-directional ammonium transport upon heterologous expression in yeast. Using yeast as an expression tool, we have started a structure-function analysis of distinct members from the Mep/Amt/Rh super-family.
