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Simple and rapid liquid chromatography method for determination of efavirenz in plasma
Geetha Ramachandran1, A K Hemanth Kumar, Soumya Swaminathan
1Tuberculosis Research Centre, Indian Council of Medical Research, Chetput, Chennai, India.
Abstract:
A simple and rapid high performance liquid chromatographic method for determination of efavirenz in human plasma was developed. The method involved extraction of sample with ethyl acetate and analysis using a reversed-phase C(18) column (150 mm) with UV detection. The assay was linear from 0.0625 to 10.0 microg/ml. The method was specific for efavirenz estimation and the drug was stable in plasma up to one month at -20 degrees C. The average recovery of efavirenz from plasma was 101%. Due to its simplicity, the assay can be used for pharmacokinetic studies and therapeutic drug monitoring of efavirenz.
