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Concurrent lipopolysaccharide enhances chemotactic response of human polymorphonuclear leukocytes to bacterial
H R Creamer1, N Hunter, W W Bullock
1Department of Oral Microbiology/Immunology, School of Dentistry, Oregon Health Sciences University, Portland 97201-3097.
Abstract:
Polymorphonuclear neutrophil (PMN) function is thought to be critical in resistance to infectious agents and this implies that the PMN must be able to migrate into, and to function in, environments that may have high levels of bacterial lipopolysaccharide (LPS). Therefore, we have evaluated the effect of LPS on the in vitro migration of PMNs. Our data reveal that the human PMN is resistant to the deleterious effects of high levels of LPS, that in high concentrations LPS is, itself, a direct chemoattractant for PMNs, and that PMN migration toward a bacterial chemotaxin is enhanced if LPS is also present. Such capabilities suggest that the PMN may be uniquely qualified to migrate into microenvironments that are rich in LPS.
Insights
Human polymorphonuclear neutrophils (PMNs) resist bacterial lipopolysaccharide (LPS) and are attracted to it. LPS enhances PMN migration, suggesting PMNs are well-equipped for LPS-rich environments.
Area of Science:
- Immunology
- Cellular Biology
Background:
- Polymorphonuclear neutrophils (PMNs) are crucial for fighting infections.
- PMN function is vital in environments with bacterial lipopolysaccharide (LPS).
Purpose of the Study:
- To investigate the in vitro effect of LPS on human PMN migration.
- To understand PMN behavior in LPS-rich microenvironments.
Main Methods:
- In vitro assessment of human PMN migration.
- Exposure of PMNs to varying concentrations of bacterial LPS.
Main Results:
- Human PMNs demonstrate resistance to high concentrations of LPS.
- High LPS levels act as a direct chemoattractant for PMNs.
- LPS enhances PMN migration towards bacterial chemotaxins.
Conclusions:
- Human PMNs possess unique capabilities to navigate and function in LPS-abundant sites.
- These findings highlight PMN adaptability in infectious microenvironments.