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Updated: Aug 5, 2026

Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays
Published on: April 7, 2015
[Effect of Congo red on the motility of the bacterium Azospirillum brasilense]
Abstract:
In semiliquid laboratory media, the bacterium Azospirillum brasilense migrates with the formation of swarming rings. It is demonstrated that adsorption of the sulfonated azodye Congo Red confers on A. brasilense the ability to consistently spread in a semiliquid agar and form microcolonies. Spontaneous variants of A. brasilense with rapid swarming are described, as well as variants that swarm in the presence of Congo Red. It is assumed that at least two types of compounds are formed, which are (a) necessary for swarming and/or spreading with the formation of microcolonies and (b) capable of interacting with Congo Red.
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