Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequential analysis of multiple analytes using a surface plasmon resonance (SPR) biosensor.

J W Chung1, R Bernhardt, J C Pyun

  • 1Korea Institute for Science and Technology Europe (KIST Europe fGmbH), Stuhlsatzenhausweg 97, 66123 Saarbruecken, Germany.

Journal of Immunological Methods
|April 4, 2006
PubMed
Summary

A novel sequential analysis method using a surface plasmon resonance (SPR) biosensor allows for the simultaneous detection of two analytes in a single sample. This method demonstrates high accuracy and no cross-reactivity, proving effective for analyzing biomarkers like human chorionic gonadotropin (hCG).

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Is there a clinical difference in paediatric congenital cholesteatoma according to age?

The Journal of laryngology and otology·2022
Same author

Bioinspired calcium phosphate mineralization on Net-Shape-Nonwoven chitosan scaffolds stimulates human bone marrow stromal cell differentiation.

Biomedical materials (Bristol, England)·2019
Same author

Characteristics and pathogenesis of facial nerve stimulation after cochlear implant surgeries: A single-centre retrospective analysis from 1151 patients.

Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery·2018
Same author

Efficiency of the sulfate pathway in comparison to the Δ4- and Δ5-pathway of steroidogenesis in the porcine testis.

The Journal of steroid biochemistry and molecular biology·2017
Same author

Effect of sulfonated steroids on steroidogenic cytochrome P450-dependent steroid hydroxylases.

The Journal of steroid biochemistry and molecular biology·2017
Same author

Anti-inflammatory effects of simvastatin in nonsteroidal anti-inflammatory drugs-induced small bowel injury.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2017

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Biosensing Technology

Background:

  • Multiplexed analysis of biological samples is crucial for accurate disease diagnosis.
  • Existing biosensing methods can face challenges with cross-reactivity and sample complexity.
  • Surface Plasmon Resonance (SPR) biosensors offer high sensitivity but require optimized methods for analyzing multiple analytes.

Purpose of the Study:

  • To develop and validate a sequential analysis method for detecting two analytes simultaneously using a single SPR biosensor.
  • To establish two distinct detection models to accommodate different analyte compositions and labeling requirements.
  • To optimize the immunoaffinity capacity for enhanced analyte binding and sensor response.

Main Methods:

  • Development of a sequential analysis protocol for a single SPR sensing region.

Related Experiment Videos

  • Design of two detection models: one with label-free and labeled detection, the other with dual labeled detection.
  • Preparation of standard curves for each model and sequential analysis of anti-bovine serum albumin (anti-BSA) antibodies and horseradish peroxidase (HRP).
  • Optimization of analyte binding capacity by adjusting molecular recognition element concentration ratios.

    • Main Results:

    • The sequential analysis method achieved errors of less than 6% for both models, indicating high accuracy.
    • No significant cross-reactivity was observed between participating antigens and antibodies in either model.
    • Optimization of immunoaffinity (IA) capacity using sensor response (R(max)) was successfully demonstrated.
    • Feasibility was confirmed by detecting human chorionic gonadotropin (hCG) and human albumin (hA) in human urine samples using Model 2.

    Conclusions:

    • The developed sequential SPR biosensor method provides a reliable and accurate approach for analyzing multiple analytes in a single sample.
    • This method minimizes cross-reactivity and allows for optimization of binding capacity, enhancing analytical performance.
    • The successful detection of pregnancy-related biomarkers (hCG and hA) in urine highlights its clinical diagnostic potential.