Simple and rapid high-performance liquid chromatographic method for determination of celecoxib in plasma using UV
A Zarghi1, A Shafaati, S M Foroutan
1Department of Pharmaceutical Chemistry, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, No. 10, Shams Alley, Across from Tavanir St., Vali-E-Asr Ave., Tehran, Iran. azarghi@yahoo.com
Abstract:
A rapid, sensitive and reproducible HPLC method was developed and validated for the analysis of celecoxib in human plasma. The analysis was carried out on a monolithic silica column using UV detection at 254 nm. The assay enables the measurement of celecoxib for therapeutic drug monitoring with a minimum quantification limit of 10 ng ml(-1). The method involves simple, one-step extraction procedure, and analytical recovery was 100.5 +/- 1.3%. The calibration curve was linear over the concentration range of 10-800 ng ml(-1). The coefficients of variation for inter-day and intra-day assay were found to be less than 8%. We also demonstrate the applicability of this method for pharmacokinetic studies in humans.
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