Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Opioid Analgesics: Morphine and Other Natural Cogeners01:20

Opioid Analgesics: Morphine and Other Natural Cogeners

1.0K
Opioids are a class of drugs that mimic endogenous opioid peptides and act on opioid receptors, and help in pain relief. These compounds are classified as natural, synthetic, or semi-synthetic. Natural opioids, like morphine, codeine, and thebaine, are derived from the opium poppy plant (Papaver somniferum or Papaver album) and are termed opiates. Synthetic opioids are artificial, while semi-synthetic opioids combine natural and synthetic compounds. Morphine, a prototypical opioid, possesses a...
1.0K
Proteomics01:33

Proteomics

9.7K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.7K
Frequency-dependent Selection01:21

Frequency-dependent Selection

24.0K
When the fitness of a trait is influenced by how common it is (i.e., its frequency) relative to different traits within a population, this is referred to as frequency-dependent selection. Frequency-dependent selection may occur between species or within a single species. This type of selection can either be positive—with more common phenotypes having higher fitness—or negative, with rarer phenotypes conferring increased fitness.
24.0K
Drug Dependence01:17

Drug Dependence

1.7K
Medications are typically administered to achieve therapeutic effects. Some drugs can modify an individual's mood and perception, frequently resulting in various enjoyable experiences. However, this can result in drug dependency, a condition marked by continuous drug use despite potential negative consequences. Drug dependency primarily falls into two categories: psychological and physical dependence. Psychological dependence occurs when the pleasurable feelings induced by the drug...
1.7K
Contact-dependent Signaling01:19

Contact-dependent Signaling

47.0K
Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
47.0K
Temperature Dependence on Reaction Rate02:55

Temperature Dependence on Reaction Rate

89.0K
The Collision Theory
Atoms, molecules, or ions must collide before they can react with each other. Atoms must be close together to form chemical bonds. This premise is the basis for a theory that explains many observations regarding chemical kinetics, including factors affecting reaction rates.
The collision theory is based on the postulates that (i) the reaction rate is proportional to the rate of reactant collisions, (ii) the reacting species collide in an orientation allowing contact between...
89.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative Measurement of Hexoses by Betaine Aldehyde Derivatisation.

International journal of molecular sciences·2026
Same author

Liver and Kidney Tissues Under Opioid Exposure: Rewriting and Old Story Through Proteomics and MALDI-MSI.

Journal of proteome research·2026
Same author

The diversity of cellular systems involved in carbonate precipitation by Escherichia coli.

PloS one·2025
Same author

Structural perspective on the design of selective DYRK1B inhibitors.

International journal of biological macromolecules·2025
Same author

Assessing Label Stability in Oligopeptide-Modified Polymer Filament for Advanced Materials: Ultraviolet Exposure and Biodegradation Study.

ACS sustainable chemistry & engineering·2025
Same author

The Role of LVV-H7 in Alcohol-Induced Reward Mechanisms.

Addiction biology·2025

Related Experiment Video

Updated: Jan 31, 2026

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

5.1K

Rat brain proteome in morphine dependence.

Anna Bierczynska-Krzysik1, Emilia Bonar, Anna Drabik

  • 1Faculty of Chemistry and Regional Laboratory, Jagiellonian University, Krakow, Poland.

Neurochemistry International
|April 4, 2006
PubMed
Summary

Researchers identified eleven potential drug dependence markers using proteomics. These proteins, including enzymes and proteasome subunits, may help understand addiction mechanisms.

More Related Videos

A Rat Model of EcoHIV Brain Infection
08:48

A Rat Model of EcoHIV Brain Infection

Published on: January 21, 2021

3.8K
Assessment of Morphine-induced Hyperalgesia and Analgesic Tolerance in Mice Using Thermal and Mechanical Nociceptive Modalities
07:23

Assessment of Morphine-induced Hyperalgesia and Analgesic Tolerance in Mice Using Thermal and Mechanical Nociceptive Modalities

Published on: July 29, 2014

34.1K

Related Experiment Videos

Last Updated: Jan 31, 2026

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

5.1K
A Rat Model of EcoHIV Brain Infection
08:48

A Rat Model of EcoHIV Brain Infection

Published on: January 21, 2021

3.8K
Assessment of Morphine-induced Hyperalgesia and Analgesic Tolerance in Mice Using Thermal and Mechanical Nociceptive Modalities
07:23

Assessment of Morphine-induced Hyperalgesia and Analgesic Tolerance in Mice Using Thermal and Mechanical Nociceptive Modalities

Published on: July 29, 2014

34.1K

Area of Science:

  • Biochemistry
  • Proteomics
  • Neuroscience

Background:

  • Drug dependence is a complex condition with significant societal impact.
  • Identifying reliable biomarkers for drug dependence is crucial for diagnosis and treatment.
  • Proteomic approaches offer a powerful tool for discovering such biomarkers.

Purpose of the Study:

  • To identify novel protein markers associated with drug dependence.
  • To utilize a proteomic strategy to compare protein expression in morphine-treated and control subjects.
  • To uncover potential molecular targets for understanding and treating drug dependence.

Main Methods:

  • Comparative proteomic analysis of samples from morphine-treated and control animals.
  • Protein separation using gel electrophoresis and precipitation techniques (TCA/acetone).
  • High-resolution protein identification via nano-scale liquid chromatography coupled to tandem mass spectrometry (nLC-MS/MS).

Main Results:

  • Eleven potential protein markers for drug dependence were identified.
  • Key identified markers include cytoplasmic and mitochondrial enzymes.
  • Notable protein families implicated are GTPase, GST, ATPase, asparaginase, and proteasome subunit p27.

Conclusions:

  • The study successfully identified potential protein biomarkers for drug dependence.
  • These identified proteins, particularly enzymes, may play significant roles in the development of addiction.
  • Further research into these markers could lead to improved diagnostic tools and therapeutic strategies for drug dependence.