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Updated: Aug 9, 2026

Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
Published on: January 30, 2019
Parallels among positive-strand RNA viruses, reverse-transcribing viruses and double-stranded RNA viruses
1Institute for Molecular Virology and Howard Hughes Medical Institute, University of Wisconsin--Madison, Madison, Wisconsin 53706, USA. ahlquist@wisc.edu
Abstract:
Viruses are divided into seven classes on the basis of differing strategies for storing and replicating their genomes through RNA and/or DNA intermediates. Despite major differences among these classes, recent results reveal that the non-virion, intracellular RNA-replication complexes of some positive-strand RNA viruses share parallels with the structure, assembly and function of the replicative cores of extracellular virions of reverse-transcribing viruses and double-stranded RNA viruses. Therefore, at least four of seven principal virus classes share several underlying features in genome replication and might have emerged from common ancestors. This has implications for virus function, evolution and control.
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