Related Experiment Video
Updated: Aug 9, 2026
Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
Published on: July 14, 2017
Direct sensitive quantitative lC/MS analysis of C-peptide from human urine by two dimensional reverse phase/reverse
Eduard Rogatsky1, Vlad Tomuta, Greg Cruikshank
1General Clinical Research Center Analytical Core Laboratory and Division of Endocrinology/Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA. rogatsky@aecom.yu.edu
Abstract:
We describe for the first time the direct quantitative analysis of human C-peptide from urine by isotope dilution assay. Implementation of 2-D reverse phase-reverse phase chromatography (2-D RP-RP) with SIM detection resulted in high assay sensitivity (LOQ= 5 pg on column), accuracy, and method ruggedness. Our experiments demonstrate the strong resolving capability of our proposed 2-D RP-RP platform which significantly reduces strong matrix effects and their resulting quantitation error over a wide range of urine concentrations. In contrast, single column methods (both SIM and multiple reaction monitoring) were found acceptable only for strongly diluted urine samples.
More Related Videos
10:17High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
08:37Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry
Published on: August 7, 2012
