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Growth retardation and lethality induced by adriblastina in chick embryo
Satya Narayan Shamal1, Shashi Kant Pandey, Bimal Kishore Panda
1Department of Anatomy, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
Insights
Adriblastina (doxorubicin) administration to chick embryos caused dose-dependent growth retardation and lethality. This study confirms adriblastina
Area of Science:
- Developmental toxicology
- Embryology
- Pharmacology
Background:
- Adriblastina (doxorubicin) is a widely used chemotherapy agent.
- Understanding its potential teratogenic effects is crucial for risk assessment.
Purpose of the Study:
- To investigate the teratogenic potential of adriblastina in developing chick embryos.
- To evaluate the dose- and time-dependent effects of adriblastina exposure during embryonic development.
Main Methods:
- Chick embryos were injected with two doses of adriblastina (1.2 mg/kg and 2.4 mg/kg) or distilled water (control) at various incubation stages.
- Embryos were collected on day 19 of gestation for analysis.
Main Results:
- Adriblastina treatment resulted in significant growth retardation and increased lethality in chick embryos.
- These effects were dose-dependent and statistically significant (p<0.001) compared to controls.
- Exposure timing also influenced the observed developmental abnormalities.
Conclusions:
- Adriblastina exhibits teratogenic effects on developing chick embryos.
- Both dosage and timing of exposure are critical factors influencing adriblastina's developmental toxicity.
Abstract:
The low (1.2 mg/kg) and high (2.4 mg/kg) therapeutic dose of adriblastina, dissolved in 0.04 ml of distilled water was injected into the chick embryo at different duration of the incubation. The control chick embryo received equal volume of distilled water at the same duration. All groups of embryo were collected on day 19 of gestation. The treated embryo showed growth retardation and lethality in a dose dependence response. The lethality and growth retardation in both treated groups were found significantly different (p<0.001) as compared with the control chick embryo. Similarly the groups treated on different days showed a time sequential effect on the developing chick embryo. Our observation had revealed that the drug is teratogenic to the chick embryo.
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