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Elevation of monocyte-derived microparticles in patients with diabetic retinopathy
Nahoko Ogata1, Shosaku Nomura, Akira Shouzu
1Department of Ophthalmology, Kansai Medical University, Moriguchi, Osaka, Japan. ogata@takii.kmu.ac.jp
Abstract:
Diabetic retinopathy is associated with microvascular damage and capillary occlusions which are common features of the microangiopathy in diabetes. Monocyte-derived microparticles (MDMPs) are released from activated monocytes and enhance the procoagulant activity, and also activate adhesion reactions. These are key events in the development of capillary occlusion. The MDMPs level in the blood, and platelet activation markers (platelet-derived microparticles (PDMPs), CD62P and CD63) were measured by flow cytometry in 72 diabetic patients. The plasma levels of intracellular adhesion molecule-1 (ICAM-1) and P-selectin were analyzed by ELISA. The level of MDMPs was significantly correlated with the levels of PDMPs (r=0.52, P<0.001), CD62P (r=0.37, P=0.001), CD63 (r=0.31 and P=0.007), P-selectin (r=0.38, P=0.001), and ICAM-1 (r=0.31, P=0.009). The MDMPs level increased with the progression of the diabetic retinopathy: 81+/-14/10(4)platelets (plts) in patients without retinopathy (n=10); 88+/-8/10(4)plts with mild or moderate non-proliferative diabetic retinopathy (NPDR, n=12); 95+/-8/10(4)plts with severe NPDR (n=24); and 112+/-9/10(4)plts with proliferative diabetic retinopathy (PDR) (n=26). The MDMPs level in patients with areas of capillary occlusion (123+/-10/10(4)plts, n=25) was significantly higher than that in patients without areas of capillary occlusion (84+/-5/10(4)plts, n=25; P=0.0008). These correlations suggest that increased levels of MDMPs may accelerate the progression of diabetic retinopathy.
Insights
Monocyte-derived microparticles (MDMPs) are elevated in diabetic retinopathy patients and correlate with disease severity and capillary occlusion, suggesting they accelerate diabetic retinopathy progression.
Area of Science:
- Endocrinology
- Vascular Biology
- Ophthalmology
Background:
- Diabetic retinopathy involves microvascular damage and capillary occlusions.
- Monocyte-derived microparticles (MDMPs) contribute to procoagulant activity and adhesion, key in capillary occlusion.
- Elevated MDMPs are implicated in the pathogenesis of diabetic microangiopathy.
Purpose of the Study:
- To investigate the association between Monocyte-derived microparticles (MDMPs) and diabetic retinopathy.
- To evaluate the correlation of MDMPs with platelet activation markers and adhesion molecules.
- To determine if MDMP levels correlate with the progression and severity of diabetic retinopathy.
Main Methods:
- Flow cytometry was used to measure MDMPs and platelet activation markers (platelet-derived microparticles (PDMPs), CD62P, CD63) in 72 diabetic patients.
- ELISA was employed to analyze plasma levels of intracellular adhesion molecule-1 (ICAM-1) and P-selectin.
- Statistical analysis correlated MDMP levels with various clinical parameters and markers.
Main Results:
- MDMP levels showed significant correlations with PDMPs, CD62P, CD63, P-selectin, and ICAM-1.
- MDMP levels increased progressively with diabetic retinopathy severity, from no retinopathy to proliferative diabetic retinopathy (PDR).
- Patients with capillary occlusion had significantly higher MDMP levels compared to those without.
Conclusions:
- Increased Monocyte-derived microparticles (MDMPs) are strongly associated with diabetic retinopathy.
- MDMP levels correlate with markers of platelet activation and adhesion molecules.
- Elevated MDMPs may play a role in accelerating the progression of diabetic retinopathy and capillary occlusion.
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