Related Experiment Videos
Liquid-liquid extraction systems for THC-COOH and benzoylecgonine
S B Needleman1, K Goodin, W Severino
1Navy Drug Screening Laboratory, Great Lakes, Illinois 60088.
Journal of Analytical Toxicology
|July 1, 1991
Summary
A new liquid-liquid extraction method is needed for military drug screening labs after a key instrument was discontinued. This method efficiently isolates THC-COOH and benzoylecgonine from urine samples.
Area of Science:
- Forensic Chemistry
- Analytical Chemistry
- Toxicology
Background:
- DuPont Instruments discontinued the PREP automated sample processor.
- Military drug screening laboratories require an alternative extraction method.
- Standard procedures for detecting THC-COOH and benzoylecgonine are impacted.
Purpose of the Study:
- To develop and validate an alternative method for extracting THC-COOH and benzoylecgonine from urine.
- To replace the discontinued automated sample processor in standard drug screening procedures.
Main Methods:
- Liquid-liquid extraction for THC-COOH using isobutanol-hexane.
- Back extraction into an aqueous alkaline medium for THC-COOH.
- Extraction into ethanol-methylene chloride for benzoylecgonine.
- Evaporation and derivatization with 1-iodopropane for benzoylecgonine.
Main Results:
- Percent recovery for THC-COOH isolation was 104.24 +/- 9.71%.
- Percent recovery for benzoylecgonine isolation was 102.43 +/- 3.13%.
Conclusions:
- The developed liquid-liquid extraction method is effective for isolating THC-COOH and benzoylecgonine.
- This method provides a viable alternative for military drug screening laboratories.
- High recovery rates indicate the method's reliability for forensic analysis.