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Updated: Aug 9, 2026

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
Published on: July 22, 2011
An allele of gyrA prevents Salmonella enterica serovar Typhimurium from using succinate as a carbon source
George E Schmitz1, Diana M Downs
1Department of Bacteriology, University of Wisconsin-Madison, 420 Henry Mall, Rm. 120, Madison, WI 53706, USA.
Abstract:
A mutant gyrA allele resulting in an A271E substitution in the DNA gyrase protein generated a strain unable to grow on the C(4)-dicarboxylates succinate, malate, and fumarate. Bacteria harboring gyrA751 displayed decreased negative supercoiling in cells. Expression of the dctA gene, which encodes the C(4)-dicarboxylate transporter, was reduced in a gyrA751 mutant, providing the first evidence that dctA expression is supercoiling sensitive and uncovering a simple metabolic screen for lesions in gyrase that reduce negative supercoiling.
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