Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

7.7K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
7.7K
Electrophoresis: Overview01:20

Electrophoresis: Overview

4.3K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
4.3K
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

116.2K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
116.2K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.5K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.5K
SDS-PAGE01:27

SDS-PAGE

34.1K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
34.1K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

1.3K
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of Memory and Active Forces on Transition Path Time Distributions.

The journal of physical chemistry. B·2018
Same author

Comparative phenology of dormant Japanese pear (Pyrus pyrifolia) flower buds: a possible cause of 'flowering disorder'.

Tree physiology·2018
Same author

Non-Markovian dynamics of reaction coordinate in polymer folding.

Soft matter·2017
Same author

Dynamical diagram and scaling in polymer driven translocation.

The European physical journal. E, Soft matter·2011
Same author

Prolonged increase of plasma non-esterified fatty acids fully abolishes the stimulatory effect of 24 hours of moderate hyperglycaemia on insulin sensitivity and pancreatic beta-cell function in obese men.

Diabetologia·2004
Same author

X-ray analyses of DNA dodecamers containing 2'-deoxy-5-formyluridine.

Nucleic acids research. Supplement (2001)·2003

Related Experiment Video

Updated: Feb 23, 2026

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

7.2K

DNA electrophoresis in designed channels.

T Sakaue1

  • 1Yukawa Institute for Theoretical Physics, Kyoto University, Kyoto 606-8502, Japan. sakaue@yukawa.kyoto-u.ac.jp

The European Physical Journal. E, Soft Matter
|April 6, 2006
PubMed
Summary

We describe polyelectrolyte movement through narrow channels, identifying three critical electric fields governing their behavior. Chain deformation at high fields enhances permeation, leading to non-monotonic electrophoretic mobility dependent on chain length.

Area of Science:

  • Polymer physics
  • Soft matter physics
  • Electrophoresis

Background:

  • Polyelectrolytes are polymers with charged groups, exhibiting complex behavior in electric fields.
  • Understanding their dynamics in confined geometries is crucial for applications in microfluidics and nanotechnology.

Purpose of the Study:

  • To elucidate the electrophoretic dynamics of polyelectrolytes in designed slit-like channels with narrow constrictions.
  • To identify critical electric fields influencing polyelectrolyte behavior and permeation.

Main Methods:

  • Analysis of rheological behaviors of polyelectrolytes under electric fields and solvent flow.
  • Theoretical description of electrophoretic dynamics in confined geometries.

Main Results:

More Related Videos

DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes
04:18

DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes

Published on: March 31, 2019

14.7K
Electroeluting DNA Fragments
06:13

Electroeluting DNA Fragments

Published on: September 5, 2010

28.5K

Related Experiment Videos

Last Updated: Feb 23, 2026

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

7.2K
DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes
04:18

DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes

Published on: March 31, 2019

14.7K
Electroeluting DNA Fragments
06:13

Electroeluting DNA Fragments

Published on: September 5, 2010

28.5K
  • Three critical electric fields (permeation, deformation, injection) were identified, dependent on the polymerization index (N).
  • Chain deformation at the slit entrance enhances permeation rate for electric fields above the deformation threshold.
  • Electrophoretic mobility shows non-monotonic dependence on N, increasing for longer chains.

Conclusions:

  • The study provides a theoretical framework for polyelectrolyte electrophoretic dynamics in constrictive channels.
  • Observed phenomena, including abrupt changes in flow, are interpreted as nonequilibrium phase transitions.
  • Results align with experimental observations for long polyelectrolyte chains.