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The maize transposable Ds1 element is alternatively spliced from exon sequences.

S R Wessler1

  • 1Botany Department, University of Georgia, Athens 30602.

Molecular and Cellular Biology
|December 1, 1991
PubMed
Summary

The maize waxy (Wx) gene

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Area of Science:

  • Plant Molecular Biology
  • Genetics
  • Transposable Elements

Background:

  • The maize waxy (Wx) gene is crucial for starch biosynthesis in endosperm.
  • The null wx-ml allele contains a Dissociation 1 (Ds1) element within exon 9.
  • Transposition of Ds elements is typically dependent on the autonomous Activator (Ac) element.

Purpose of the Study:

  • To investigate the molecular mechanisms underlying transcript generation from the wx-ml allele.
  • To characterize the splicing patterns of Ds1 sequences within the Wx pre-mRNA.
  • To explore the potential of Ds1 as a mobile intron.

Main Methods:

  • Analysis of Wx transcripts from the wx-ml allele in the absence of Ac.
  • Identification and characterization of alternative splice sites within the Ds1 element.
  • Comparative analysis of Ds1 features with other known Ds elements.

Main Results:

  • The Ds1 element in the wx-ml allele is not excised in the absence of Ac.
  • Alternative splicing of Ds1 sequences generates multiple Wx transcripts.
  • Three 5' and three 3' splice sites are utilized within the Ds1 element.
  • Ds1 exhibits unique features, including splice sites near its termini, small size, and AT richness.

Conclusions:

  • The Ds1 element in the wx-ml allele functions as a mobile intron due to its specific sequence and splice site characteristics.
  • These features facilitate alternative splicing, leading to diverse Wx transcripts.
  • Ds1 represents a distinct class of Ds elements with potential implications for gene regulation and genome evolution in maize.

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