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Adaptation by Streptococcus mutans to acid tolerance.

I R Hamilton1, N D Buckley

  • 1University of Manitoba, Winnipeg, Canada.

Oral Microbiology and Immunology
|April 1, 1991
PubMed
Summary
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Streptococcus mutans adapts to acidic environments by shifting its pH optimum for glucose uptake and glycolysis. This adaptation allows the bacteria to maintain essential functions even at lower pH levels.

Area of Science:

  • Microbiology
  • Oral Health
  • Bacterial Physiology

Background:

  • Streptococcus mutans demonstrates adaptability to acidic conditions.
  • Previous continuous culture studies suggested this capacity.
  • Further investigation into acid adaptation mechanisms is warranted.

Purpose of the Study:

  • To investigate the detailed acid adaptation of Streptococcus mutans.
  • To determine the shift in pH optima for glucose uptake and glycolysis.
  • To assess the capacity of Streptococcus mutans to generate pH gradients under acidic conditions.

Main Methods:

  • Continuous culture of S. mutans Ingbritt and a phosphotransferase system (PTS)-defective mutant (S. mutans DR0001/6) at varying pH levels (7.5, 5.5, 5.1).
  • Determination of pH optima for glucose uptake and glycolysis using steady-state, washed cells.

Related Experiment Videos

  • Measurement of proton permeability via an acid pulse technique.
  • Assessment of pH gradient generation capacity.

    • Main Results:

    • The pH optimum for glucose uptake shifted to lower values in cells adapted to acidic environments.
    • A similar shift was observed for glycolysis, particularly when proton gradients were dissipated.
    • Acid-adapted cells maintained pH gradients at lower pH, with the PTS-defective mutant showing a greater capacity.

    Conclusions:

    • Streptococcus mutans exhibits significant physiological adaptation to acidic environments.
    • The phosphotransferase system (PTS) plays a role in the acid tolerance of Streptococcus mutans.
    • These findings contribute to understanding bacterial survival strategies in low-pH conditions relevant to oral health.