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Differences in the two-dimension-gel electrophoresis protein patterns of the lethal K and benign B variants of

D R Cerutis1, D J Giron

  • 1Department of Pharmacology, University of Nebraska Medical Center, Omaha 68105.

Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
|December 1, 1991
PubMed

Insights

Encephalomyocarditis virus (EMCV) variants cause different diseases despite similar antigenicity. Protein analysis revealed distinct viral proteins in the lethal EMCV-K strain compared to the benign EMCV-B strain, offering insights into pathogenicity.

Area of Science:

  • Virology
  • Molecular Biology
  • Pathogenesis research

Background:

  • Encephalomyocarditis virus (EMCV) variants exhibit antigenic similarity but cause diverse disease syndromes in mice.
  • Understanding the molecular basis for differential pathogenicity among EMCV strains is crucial.

Purpose of the Study:

  • To characterize and compare the protein profiles of a benign EMCV variant (EMCV-B) and a lethal EMCV variant (EMCV-K).
  • To identify viral protein differences that may explain the distinct pathogenicity of EMCV strains.

Main Methods:

  • Two-dimensional gel electrophoresis was employed to compare protein expression between EMCV-B and EMCV-K.
  • Analysis focused on identifying differences in viral proteins, including capsid proteins like VP1.

Main Results:

  • Both EMCV variants demonstrated replication cycles typical of picornaviruses.
  • The viral protein 1 (VP1) of EMCV-K was found to be more basic than that of EMCV-B.
  • Three unique proteins, including a major VP1 component and two ~12,000 molecular weight proteins, were detected in EMCV-K but absent in EMCV-B.

Conclusions:

  • Distinct protein profiles, particularly variations in VP1 and the presence of specific unique proteins, correlate with the differential pathogenicity of EMCV variants.
  • These protein differences are key factors contributing to the varied disease outcomes observed between EMCV-B and EMCV-K infections.

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