Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Carotenoid transformations in the chloroplast envelope.

S W Jeffrey1, R Douce, A A Benson

  • 1Scripps Institution of Oceanography, University of California, San Diego, La Jolla, Calif. 92037.

Proceedings of the National Academy of Sciences of the United States of America
|March 1, 1974
PubMed
Summary

Spinach chloroplast envelopes exhibit unique carotenoid compositions that change with light exposure. Violaxanthin de-epoxidation is a light-catalyzed process influencing chloroplast envelope function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hospital management of colonic perforations complicating ambulatory outpatient colonoscopy via over-the-scope clips or surgery: a case series.

Techniques in coloproctology·2019
Same author

EMILIANIA HUXLEYI (HAPTOPHYTA) HOLDS PROMISING INSIGHTS FOR PHOTOSYNTHESIS.

Journal of phycology·2018
Same author

PAH fluxes to Siskiwit revisted: trends in fluxes and sources of pyrogenic PAH and perylene constrained via radiocarbon analysis.

Environmental science & technology·2013
Same author

The role of vitamin D in the immunopathogenesis of allergic skin diseases.

Allergy·2011
Same author

Strongyloidiasis presenting as eosinophilic ascites.

Annals of tropical medicine and parasitology·2011
Same author

Preparation of 7-chloro-4-(2-piperidylmethylamino)-quinoline.

Journal of the American Chemical Society·2010

Area of Science:

  • Plant Biology
  • Photosynthesis Research
  • Chloroplast Ultrastructure

Background:

  • Chloroplast envelopes possess a distinct carotenoid composition.
  • Carotenoids play crucial roles in photosynthesis and photoprotection.
  • Light-induced biochemical changes within chloroplasts are actively studied.

Purpose of the Study:

  • To investigate the carotenoid composition of spinach chloroplast envelopes.
  • To determine the effect of light exposure on violaxanthin, lutein, and zeaxanthin levels.
  • To explore the functional significance of light-catalyzed carotenoid changes in chloroplast envelopes.

Main Methods:

  • Isolation and preparation of spinach chloroplast envelopes.
  • Spectrophotometric analysis of carotenoid content.

Related Experiment Videos

  • Comparison of carotenoid ratios in dark-treated versus illuminated leaf samples.

    • Main Results:

    • Chloroplast envelopes from dark-treated spinach leaves showed a violaxanthin content 3.5 times higher than lutein plus zeaxanthin.
    • In contrast, envelopes from illuminated leaves had a violaxanthin to lutein plus zeaxanthin ratio of 0.75.
    • Light-catalyzed changes in violaxanthin content were also observed in the thylakoid fraction.

    Conclusions:

    • The reversible light-catalyzed de-epoxidation of violaxanthin is a key process in chloroplast envelopes.
    • This process likely plays a significant role in the functional adaptation of chloroplast envelopes to varying light conditions.
    • Further research is needed to fully elucidate the precise function of this mechanism.