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Updated: Aug 9, 2026

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
Published on: January 4, 2017
Thermosensitive step associated with transfer of the Ti plasmid during conjugation: Possible relation to
1Génétique et Amélioration des Plantes, Institut National de la Recherche Agronomique, 78000 Versailles, France.
Abstract:
It is reported here that transfer by means of a conjugative process of an oncogenic plasmid from a virulent strain of Agrobacterium tumefaciens to a strain of that organism that had been cured of the plasmid is thermosensitive. Since the thermosensitive step found in the conjugative process appears similar in every respect to a thermosensitive step that is involved in the transformation of a normal cell to a tumor cell in the crown gall disease of plants, it is suggested that the observed results may reflect the existence of a thermosensitive step that is common to these two processes.
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