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ATP-dependent Ca uptake into plant membrane vesicles.

J Gross1, D Marmé

  • 1Institut für Biologie III, University of Freiburg, 7800 Freiburg, Germany.

Proceedings of the National Academy of Sciences of the United States of America
|March 1, 1978
PubMed
Summary

Plant membrane vesicles actively transport calcium ions (Ca2+) using adenosine triphosphate (ATP) and magnesium (Mg2+). Oxalate enhances Ca2+ accumulation, indicating a potential role in plant calcium storage.

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Area of Science:

  • Plant Physiology
  • Cell Biology
  • Biochemistry

Background:

  • Understanding calcium ion (Ca2+) transport mechanisms is crucial for plant cell function.
  • Membrane vesicles are key components in cellular transport processes.

Purpose of the Study:

  • To investigate the active Ca2+ uptake mechanism in plant membrane vesicles.
  • To characterize the factors influencing Ca2+ transport and accumulation.

Main Methods:

  • Extraction of membrane vesicles from various plant sources (etiolated and light-grown plants, cell suspension culture, alga).
  • Assay of Ca2+ uptake in the presence of adenosine triphosphate (ATP) and magnesium (Mg2+).
  • Analysis of Ca2+ efflux and the effect of oxalate, ionophores, and oligomycin.

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Main Results:

  • Demonstrated ATP- and Mg2+-dependent active Ca2+ uptake against a concentration gradient.
  • Characterized the dependence of uptake on ATP/Mg2+ concentrations, pH, and temperature.
  • Observed enhanced Ca2+ accumulation in the presence of oxalate and rapid release with ionophores; efflux was ATP-independent.

Conclusions:

  • Plant membrane vesicles possess an ATP-dependent mechanism for active Ca2+ transport.
  • Oxalate plays a significant role in promoting sustained Ca2+ accumulation.
  • This system is distinct from passive efflux mechanisms and is not directly inhibited by oligomycin.