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Updated: Jul 26, 2026
08:17
VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
Published on: August 23, 2013
Direct selection in vitro for herbicide-resistant mutants of Nicotiana tabacum
1Department of Plant Breeding and Biometry and Section of Botany, Genetics, and Development, Cornell University, Ithaca, New York 14853.
Abstract:
Seven cell lines of Nicotiana tabacum resistant to the herbicide picloram were isolated from cell cultures. In crosses of plants regenerated from four cell lines, resistance was inherited as a single dominant Mendelian allele. Plants could not be regenerated from one cell line, and expression of resistance proved unstable in two others.
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01:16In-vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
02:50Transgenic Plants
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...