Iron-dextran antibody conjugates: General method for simultaneous staining of two components in high-resolution
A H Dutton1, K T Tokuyasu, S J Singer
1Department of Biology, University of California at San Diego, La Jolla, California 92093.
Abstract:
We report the preparation and properties of an electron-dense antibody conjugate, made by the covalent bonding of an iron-dextran (Imposil) particle to an antibody molecule. Transmission electron microscopic experiments with the Imposil-antibody conjugates demonstrate their suitability as specific immunostains at high resolution, particularly for simultaneous double staining experiments in conjunction with ferritin-antibody conjugates.
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