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Related Experiment Videos

RNA intermediates in potato spindle tuber viroid replication.

R A Owens1, T O Diener

  • 1Plant Virology Laboratory, Plant Protection Institute, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705.

Proceedings of the National Academy of Sciences of the United States of America
|January 1, 1982
PubMed
Summary

Researchers identified double-stranded RNA intermediates in potato spindle tuber viroid (PSTV) replication. These findings support a rolling circle model for viroid replication, involving complementary RNA strands complexed with PSTV RNA.

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[Molecular properties of potato spindle tuber viroid (PSTVd) isolates from the collection of the Russian Research Institute of Phytopathology].

Molekuliarnaia biologiia·2013

Area of Science:

  • Plant Pathology
  • Molecular Biology
  • Virology

Background:

  • Viroids, such as potato spindle tuber viroid (PSTV), are small, infectious RNA molecules that cause plant diseases.
  • The replication mechanism of viroids remains incompletely understood, particularly the nature of intermediate RNA species.

Purpose of the Study:

  • To isolate and characterize double-stranded RNA intermediates involved in PSTV replication.
  • To elucidate the role of these intermediates in the PSTV life cycle.
  • To evaluate existing models of viroid replication.

Main Methods:

  • Isolation of RNA from PSTV-infected tomato tissue.
  • Characterization using polyacrylamide gel electrophoresis.
  • Analysis via DNA-RNA hybridization techniques.

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Main Results:

  • Two distinct double-stranded RNA intermediates were identified.
  • These intermediates consist of monomeric PSTV RNA (circular or linear) complexed with multimeric complementary RNA strands.
  • A significant increase in double-stranded PSTV RNA was observed concurrently with single-stranded PSTV synthesis.

Conclusions:

  • The characterized double-stranded RNAs are likely in vivo precursors crucial for PSTV replication.
  • A "rolling circle" replication model, utilizing a circular PSTV template, can explain the formation of these double-stranded RNA species.