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Updated: Aug 9, 2026

Optical Clearing of Plant Tissues for Fluorescence Imaging
Published on: January 5, 2022
Optical properties of etiolated plant tissues
1Department of Plant Biology, Carnegie Institution of Washington, 290 Panama Street, Stanford, California 94305.
Abstract:
Etiolated tissues of several plants are multiple bundles of fiber optics capable of coherent transfer of light over at least 20 mm. The acceptance angles (the angles at which light can be intercepted and then internally reflected longitudinally) for mung beans, oats, and corn are 47 degrees , 59 degrees , and 52 degrees -54 degrees , respectively. The shapes of the curves that describe the acceptance angles are the same for various tissues of the same plant but differ between species. The pattern of light transmitted longitudinally through a tissue is dependent on the angle at which the light intercepts the side of the tissue and is strongly influenced by the tissue geometry. When 0.5 mm of the tip is irradiated, the amount of light traveling down the "shaded" side of the coleoptile is equal to or 2- to 3-fold greater than the amount traveling down the "lighted" side.
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