Tumor induction by Agrobacterium rhizogenes involves the transfer of plasmid DNA to the plant genome
F F White1, G Ghidossi, M P Gordon
1Department of Microbiology and Immunology, University of Washington, Seattle, Washington 98195.
Abstract:
The DNA from tumors of Nicotiana glauca initiated by strains of Agrobacterium rhizogenes was shown to contain sequences that are homologous to the root-inducing (Ri) plasmid of the bacterium. Two independently established tumor lines contained a similar portion of the Ri-plasmid. The Ri-plasmid also hybridized to DNA fragments from uninfected N. glauca. A cosmid clone of the Ri-plasmid encompassing the region containing the Ri-plasmid sequences that are stably transferred to the plant also hybridized to the Ri-plasmid-related fragments found in uninfected plants. Five of six tumor lines tested produced a tumor-specific compound that is similar to agropine.
More Related Videos
08:12Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)
Published on: March 11, 2020
11:16A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling
Published on: July 22, 2017
Related Concept Videos
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Transgenic Organisms
Bacterial Transformation
Transduction
Plant Tissue Culture
Transformation
