Related Experiment Video
Updated: Aug 9, 2026

Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota
Published on: May 23, 2025
Uridine diphosphate glucose breakdown is mediated by a unique enzyme activated by fructose 2,6-bisphosphate in
1Frito-Lay Research, 900 N, Loop 12, Irving, Texas 75061.
Abstract:
In the presence of inorganic phosphate, uridine 5'-diphosphate glucose (UDPG) is specifically hydrolyzed to glucose 1-phosphate and UDP by a unique enzyme, UDPG phosphorylase. The activity of the enzyme was maximally stimulated by fructose 2,6-bisphosphate, a regulatory metabolite recently discovered in both plants and animals, and by 2-phosphoglyceric acid. At 1 muM, fructose 2,6-bisphosphate stimulated UDPG phosphorolysis 10-fold, whereas 2-phosphoglyceric acid was required at higher concentrations (100 muM) to produce a similar effect. Fructose 2,6-bisphosphate appears to increase the affinity of the enzyme for inorganic phosphate, with a change in K(m) from 1.6 mM to 0.3 mM. The results suggest that fructose 2,6-bisphosphate participates in the regulation of other pathways of carbohydrate metabolism in addition to playing its recognized role in glycolysis and gluconeogenesis.
Related Concept Videos
Biosynthesis of Polysaccharides
Glycolysis: Preparatory Phase
Energy-requiring Steps of Glycolysis
Other Glycolytic Pathways
Glycolysis
What is Glycolysis?
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...

