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Related Experiment Videos

X-ray diffraction by phospholipid monolayers on single-crystal silicon substrates.

M Seul1, P Eisenberger, H M McConnell

  • 1Stauffer Laboratory for Physical Chemistry, Department of Chemistry, Stanford University, Stanford, California 94305.

Proceedings of the National Academy of Sciences of the United States of America
|September 1, 1983
PubMed
Summary

Supported phospholipid monolayers exhibit exceptional crystalline order, with diffraction studies revealing a phase transition around 65°C. This research advances understanding of lipid self-assembly on solid surfaces.

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Contrast inversion in the epifluorescence of cholesterol-phospholipid monolayers.

Biophysical journal·2004

Area of Science:

  • Materials Science
  • Biophysics
  • Surface Chemistry

Background:

  • Phospholipid monolayers are crucial in biological membranes and nanotechnology.
  • Studying their structure on solid supports is challenging due to substrate interference.

Purpose of the Study:

  • To investigate the structural order of dipalmitoyl phosphatidylcholine (DPPC) monolayers on silicon wafers.
  • To characterize the properties of supported lipid monolayers using advanced diffraction techniques.
  • To identify phase transitions in supported DPPC monolayers.

Main Methods:

  • Transferring DPPC monolayers from an air-water interface to octadecyltrichlorosilane-alkylated silicon wafers.
  • Utilizing synchrotron radiation for X-ray diffraction analysis.
  • Performing preliminary temperature-dependent scans.

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Main Results:

  • Observed diffraction signals at Bragg spacings of 0.4247 ± 0.0002 nm and 0.4253 ± 0.0002 nm.
  • Demonstrated high in-plane order with positional coherence length ≥ 500 nm and orientational order < 0.01 degrees.
  • Identified a phase transition at approximately 65°C.

Conclusions:

  • Supported DPPC monolayers can achieve high crystalline order.
  • Synchrotron X-ray diffraction is effective for studying such systems with minimal background scattering.
  • The observed phase transition provides insights into lipid behavior on solid substrates.