Enveloped double-stranded DNA insect virus with novel structure and cytopathology
1Division of Biological Control, Department of Entomology, University of California, Riverside, CA 92521.
Summary
A novel virus causing fatal disease in cabbage looper larvae was identified. This unique enveloped DNA virus replicates within host cells, leading to opaque white hemolymph and a previously unobserved viral group.
Area of Science:
- Virology
- Insect Pathology
- Molecular Biology
Background:
- Isolation of a novel virus from cabbage looper (Trichoplusia ni) larvae.
- The virus causes a chronic, fatal disease affecting multiple tissues.
Purpose of the Study:
- To characterize a newly isolated virus from Trichoplusia ni.
- To investigate the unique structural and developmental properties of this virus.
Main Methods:
- Isolation and transmission studies in Trichoplusia ni and other Noctuidae species.
- Electron microscopy for virion structure and replication analysis.
- Biochemical analysis of viral DNA.
Main Results:
- Viral replication involves nuclear hypertrophy, nuclear envelope rupture, and formation of viral-induced membranes.
- Vesicles containing virions accumulate in hemolymph, causing opaque white blood.
- Virions are large, enveloped, allantoid, double-stranded DNA viruses with unique hexagonal envelope subunits.
Conclusions:
- The identified virus represents a novel group of enveloped, double-stranded DNA viruses.
- Its unique replication strategy and structure distinguish it from previously known viruses.
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