Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Periodic structures in lipid monolayer phase transitions.

H M McConnell1, L K Tamm, R M Weis

  • 1Stauffer Laboratory for Physical Chemistry, Stanford University, Stanford, CA 94305.

Proceedings of the National Academy of Sciences of the United States of America
|May 1, 1984
PubMed
Summary

Supported lipid monolayers with fluorescent probes exhibit periodic patterns. These patterns arise from ordered solid lipid domains in equilibrium with fluid lipid phases, potentially stabilized by electrostatic forces.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Baseline hydrocarbon levels in New Zealand coastal and marine avifauna.

Marine pollution bulletin·2015
Same author

Spin-labeled membranes.

The Journal of general physiology·2009
Same author

Contrast inversion in the epifluorescence of cholesterol-phospholipid monolayers.

Biophysical journal·2004
Same author

The receptor docking segment and S-adenosyl-L-homocysteine bind independently to the methyltransferase of bacterial chemotaxis.

Biochimica et biophysica acta·2002
Same author

Biophysical approaches to membrane protein structure determination.

Current opinion in structural biology·2002
Same author

Condensed complexes and the calorimetry of cholesterol-phospholipid bilayers.

Biophysical journal·2001

Area of Science:

  • Biophysics
  • Materials Science
  • Surface Chemistry

Background:

  • Lipid monolayers are fundamental models for biological membranes.
  • Understanding lipid domain formation and ordering is crucial for membrane biophysics.
  • Epi-fluorescence microscopy is a key technique for visualizing lipid structures.

Purpose of the Study:

  • To investigate the formation of periodic patterns in supported lipid monolayers.
  • To determine the conditions under which these patterns emerge.
  • To explore the role of electrostatic forces in stabilizing lipid domain ordering.

Main Methods:

  • Preparation of dipalmitoyl phosphatidylcholine lipid monolayers.
  • Supported monolayers on air-water interfaces and alkylated glass coverslips.

Related Experiment Videos

  • Observation using epi-fluorescence microscopy with fluorescent lipid probes.

    • Main Results:

    • Periodic patterns were observed in doped lipid monolayers.
    • Patterns consist of periodic arrays of solid-phase lipid domains.
    • Domain ordering occurs in equilibrium with fluid-phase lipid under specific temperature and pressure conditions.

    Conclusions:

    • Periodic pattern formation is a characteristic behavior of certain lipid monolayers.
    • Electrostatic forces are proposed as a mechanism for stabilizing the observed periodic ordering.
    • These findings contribute to understanding lipid self-assembly and membrane organization.