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Updated: Aug 4, 2026
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From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
Published on: January 13, 2017
Identification of a cloned cytokinin biosynthetic gene
G F Barry1, S G Rogers, R T Fraley
1Monsanto Company, 800 N. Lindbergh Boulevard, St. Louis, MO 63167.
Abstract:
A small region of the Ti plasmid (the tmr locus), thought to be involved in phytohormone metabolism in Agrobacterium tumefaciens-transformed plant tissue, was cloned and expressed in Escherichia coli. By enzyme assay, the tmr locus was shown to encode isopentenyltransferase, an enzyme that catalyzes the first step in cytokinin biosynthesis.
