Jove
Visualize
Contact Us

Related Experiment Videos

Phorbol ester cardiotoxicity: are .O2-radicals involved?

E Esser1, G Loschen, L Flohé

  • 1Grünenthal GmbH, Center of Research, Aachen.

Archives of Toxicology
|January 1, 1991
PubMed
Summary

Superoxide dismutase (SOD) did not affect the cardiotoxic effects of phorbol 12-myristate 13-acetate (PMA) in rat hearts. This suggests PMA-induced cardiotoxicity is not mediated by superoxide radicals.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preparation of orodispersible minitablets containing a poorly compactable drug substance by aqueous fluid bed layering onto coprocessed excipients.

International journal of pharmaceutics·2026
Same author

Incorporation of micronized poorly soluble drug into orodispersible tablets by aqueous fluid bed granulation of co-processed excipients.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2026
Same author

[Pembrolizumab (Keytruda®) associated ocular hypotony].

Die Ophthalmologie·2023
Same author

[Occurrence of corneal microcystoid alterations under administration of belantamab mafodotin for multiple myeloma].

Die Ophthalmologie·2022
Same author

A dual attack on the peroxide bond. The common principle of peroxidatic cysteine or selenocysteine residues.

Redox biology·2020
Same author

Beta2-microglobulin stimulates osteoclast formation.

Kidney international·2008
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Cardiology
  • Biochemistry
  • Pharmacology

Background:

  • Phorbol 12-myristate 13-acetate (PMA) can induce cardiotoxicity.
  • Superoxide radicals are implicated in various cardiovascular pathologies.
  • The role of superoxide radicals in PMA-induced cardiotoxicity requires clarification.

Purpose of the Study:

  • To investigate the effect of superoxide dismutase (SOD) on PMA-induced cardiotoxicity in isolated perfused rat hearts.
  • To determine if superoxide radicals mediate the cardiotoxic effects of PMA.

Main Methods:

  • Isolated perfused rat hearts were used.
  • Hearts were treated with PMA +/- SOD.
  • Cardiovascular parameters (inotropic effect, vasoconstriction, bradycardia) were monitored.
  • Cytochrome c reduction in perfusate was measured as an indicator of radical formation.

Main Results:

  • SOD did not alter the negative inotropic effect, vasoconstriction, or bradycardia induced by PMA.
  • No evidence of superoxide radical formation in response to PMA was found with SOD treatment.
  • PMA-induced cytochrome c reduction was not inhibited by SOD, indicating an O2-independent mechanism.

Conclusions:

  • Superoxide dismutase does not protect against PMA-induced cardiotoxicity in isolated rat hearts.
  • The cardiotoxic effects of PMA are likely mediated by mechanisms independent of superoxide radicals.
  • Further research is needed to elucidate the specific pathways involved in PMA-induced cardiotoxicity.

Related Experiment Videos