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Related Experiment Videos

Site-specific DNA inversion is enhanced by a DNA sequence element in cis.

H E Huber1, S Iida, W Arber

  • 1Biozentrum of the University of Basel, Department of Microbiology, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.

Proceedings of the National Academy of Sciences of the United States of America
|June 1, 1985
PubMed
Summary

Bacteriophage P1 genome inversion requires a cis-acting enhancer sequence within the cin gene for efficient recombination. This enhancer significantly stimulates the site-specific recombination process, impacting phage host range.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • Bacteriophage P1's C segment undergoes inversion via site-specific recombination, altering phage host range.
  • This inversion is catalyzed by the Cin recombinase, encoded by the cin gene.

Purpose of the Study:

  • To investigate the requirements for efficient Cin-catalyzed site-specific recombination in bacteriophage P1.
  • To identify and characterize any cis-acting elements that enhance the inversion process.

Main Methods:

  • Utilized tester plasmids to measure Cin-catalyzed recombination in trans by monitoring antibiotic resistance gene expression.
  • Performed genetic analysis to identify and localize cis-acting sequences essential for efficient inversion.

Main Results:

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  • An essential cis-acting sequence, distinct from crossover sites, was identified within the cin structural gene.
  • This enhancer sequence stimulates recombination over 100-fold.
  • The enhancer's activity was largely independent of its orientation and position relative to the crossover sites.

Conclusions:

  • A novel cis-acting enhancer element within the cin gene is crucial for efficient bacteriophage P1 C segment inversion.
  • This enhancer significantly boosts the activity of the Cin recombinase, highlighting its importance in regulating phage genome plasticity and host range.