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DNA amplification patterns in maize endosperm nuclei during kernel development.

R V Kowles1, R L Phillips

  • 1Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55108.

Proceedings of the National Academy of Sciences of the United States of America
|October 1, 1985
PubMed
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DNA levels in Zea mays endosperm nuclei increase significantly during development, peaking around 14-18 days post-pollination. This DNA amplification, possibly polytenization, is crucial for endosperm growth.

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Genetics

Background:

  • Endosperm development in Zea mays is a complex process.
  • Nuclear size and DNA content are key indicators of developmental stages.

Purpose of the Study:

  • To investigate the relationship between DNA levels and endosperm development in Zea mays.
  • To characterize DNA content changes during endosperm maturation.

Main Methods:

  • Feulgen cytophotometry was used to measure DNA content per nucleus.
  • Comparative analysis across different Zea mays strains and mutant lines.
  • Cytological studies examined chromatin structure and organization.

Main Results:

  • DNA content per nucleus sharply increases, reaching peak levels of 90C to over 690C in some strains.

Related Experiment Videos

  • Mitotic activity decreases as DNA levels rise, suggesting DNA amplification rather than cell division.
  • Endosperm mutant strains, including opaque-2, showed altered DNA accumulation patterns.
  • A decrease in DNA level was observed in older endosperms.

    • Conclusions:

    • A form of DNA amplification, potentially polytenization, occurs during Zea mays endosperm development.
    • DNA accumulation patterns are consistent across growing seasons and vary among strains.
    • Mutations and genetic backgrounds influence DNA levels in endosperm nuclei.