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Updated: Aug 19, 2026
Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
Published on: January 28, 2019
Production and characterization of monoclonal antibodies against an avian group A rotavirus
1Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
Abstract:
Fifteen monoclonal antibodies (MAbs) against an avian group A rotavirus were cloned and characterized. Eight of the 15 MAbs had neutralizing activity (N-MAbs). Five of the N-MAbs (1G1, 5B8, 4E2, 3G1, 2E3) were VP4-specific by radioimmunoprecipitation assay (RIPA), and two N-MAbs (2D11, 6E8) were possibly VP7-specific (faint bands by RIPA). One N-MAb (4H12) of undefined protein specificity cross-reacted with serotype 3 simian rotaviruses. The other seven N-MAbs did not cross-react with any of the eight distinct serotypes of human and mammalian rotaviruses tested. Of the seven non-neutralizing MAbs, three were VP6-specific (3H10, 4B12, 5F6), two were VP8-specific (6C9, 1D1), one was VP4-specific (4E9), and one was of undefined protein specificity (1B11). Four non-neutralizing MAbs recognized only avian group A rotavirus in cell-culture immunofluorescence tests (6C9, 1D1, 4E9 and 5F6), whereas two MAbs (3H10 and 4B12) cross-reacted with all human and animal rotaviruses tested. The MAb 1B11 did not recognize any human rotavirus serotypes but cross-reacted with all nonhuman animal rotavirus serotypes. The MAbs produced in this study should be useful for the detection and further characterization of avian group A rotaviruses.
Insights
This study cloned and characterized fifteen monoclonal antibodies (MAbs) against avian rotavirus. Eight MAbs showed neutralizing activity, aiding in the detection and characterization of avian group A rotaviruses.
Area of Science:
- Virology
- Immunology
- Molecular Biology
Background:
- Avian rotaviruses are significant pathogens in poultry.
- Monoclonal antibodies (MAbs) are crucial tools for viral detection and characterization.
- Specific MAbs targeting avian rotavirus proteins are needed for diagnostic and research purposes.
Purpose of the Study:
- To clone and characterize monoclonal antibodies (MAbs) against avian group A rotavirus.
- To determine the neutralizing activity and protein specificity of generated MAbs.
- To assess the cross-reactivity of MAbs with human and mammalian rotaviruses.
Main Methods:
- Cloning and characterization of fifteen MAbs against avian group A rotavirus.
- Radioimmunoprecipitation assay (RIPA) to determine MAb specificity for viral proteins (VP4, VP7, VP6, VP8).
- Cell-culture immunofluorescence tests to evaluate MAb reactivity and cross-reactivity with different rotavirus strains.
Main Results:
- Eight of fifteen MAbs exhibited neutralizing activity against avian rotavirus.
- Five neutralizing MAbs were VP4-specific, and two were potentially VP7-specific.
- Seven non-neutralizing MAbs were specific for VP6, VP8, or VP4, with some showing broad cross-reactivity with human and animal rotaviruses.
Conclusions:
- The characterized MAbs, particularly neutralizing ones, are valuable reagents for avian rotavirus detection.
- Specific MAbs targeting VP4 and VP7 can differentiate avian rotaviruses.
- The study provides a panel of MAbs for further research into avian rotavirus pathogenesis and diagnostics.
