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Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
Published on: August 17, 2009
Colony formation from mesophyll protoplasts of a cereal, oat
1Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, 12, rue du Général Zimmer, F-67000 Strasbourg, France.
Abstract:
Differentiated leaf cells of gramineous plants, among them the cereals with their immense importance for human nutrition, are considered extremely recalcitrant to, if not incapable of, reentering the cell cycle. This recalcitrance is related to the poor wound response of the monocots-in contrast to most dicots-and the difficulties encountered in monocot tissue culture. We report here the highly reproducible induction of sustained divisions at high frequency (up to 95%) and colony formation from mesophyll protoplasts of a cereal, oat, demonstrating that-contrary to most earlier evidence-mesophyll cells of a gramineous plant have not irreversibly lost their potential for cell division.
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