Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Engineering RNA-based circuits.

R Narayanaswamy1, A D Ellington

  • 1Institute for Cellular and Molecular Biology, University of Texas at Austin, 1 University Station A4800, Austin TX, 78712-0159, USA.

Handbook of Experimental Pharmacology
|April 6, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "An amino acid depleted cell-free protein synthesis system for the incorporation of non-canonical amino acid analogs into proteins" [J. Biotechnol. 178 (2014) 12-22].

Journal of biotechnology·2016
Same author

A stochastic DNA walker that traverses a microparticle surface.

Nature nanotechnology·2015
Same author

Using RecA protein to enhance kinetic rates of DNA circuits.

Chemical communications (Cambridge, England)·2015
Same author

Single mode tapered fiber-optic interferometer based refractive index sensor and its application to protein sensing.

Optics express·2014
Same author

Molecularly imprinted polymers as optical sensing receptors: correlation between analytical signals and binding isotherms.

Analytica chimica acta·2011
Same author

Highly sensitive optical humidity probe.

Talanta·2008

Engineered nucleic acids offer versatile tools for cellular metabolism interventions. Combining these RNA tools into logical circuits enhances their function for biotechnological and medical applications.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Synthetic Biology

Background:

  • Nucleic acids possess diverse functions including gene modulation via base-pairing, molecular recognition, and catalysis.
  • The engineering of nucleic acids, particularly RNA, leverages Watson-Crick base-pairing for creating novel molecular tools.

Purpose of the Study:

  • To explore the potential of engineered nucleic acids as modular tools for biotechnological and medical applications.
  • To detail the design and function of genetic circuits for controlling cellular processes.
  • To anticipate future complex circuits with medical relevance.

Main Methods:

  • Engineering nucleic acid-based molecular tools utilizing Watson-Crick base-pairing principles.
  • Designing and constructing logical and regulatory genetic circuits.

Related Experiment Videos

  • Evolving genetic circuits for optimized function.
  • Detailing examples of genetic circuits controlling transcription and translation.

    • Main Results:

    • Demonstrated the modularity of engineered nucleic acids for diverse cellular functions.
    • Presented examples of functional genetic circuits for gene expression control.
    • Highlighted the potential for combining individual RNA tools into sophisticated circuits.

    Conclusions:

    • Engineered nucleic acids provide a powerful platform for developing molecular tools.
    • Integrating these tools into genetic circuits significantly enhances their utility.
    • Future development of complex circuits holds promise for advanced medical interventions in cellular metabolism.