[Experimental research on the cold-constitution and the heat-constitution (I)]

Area of Science:

• Physiology
• Biochemistry
• Endocrinology

Background:

• Individual variations in physiological responses exist, often categorized by constitutional types like cold-constitution (CC) and heat-constitution (HC).
• Understanding the underlying biochemical and hormonal differences in these constitutional types is crucial for personalized medicine and physiology research.

Purpose of the Study:

• To experimentally investigate and compare the metabolic and hormonal profiles of Wistar rats exhibiting cold-constitution (CC) and heat-constitution (HC).
• To identify specific biochemical markers and hormonal differences that characterize CC and HC phenotypes.

Main Methods:

• Selected Wistar rats representing CC and HC groups.
• Measured adenylate kinase (ADK) activities, energy charge, and Na(+)-K(+)-ATPase activities in liver tissue.
• Quantified serum levels of thyroid hormones (T3, T4), progesterone, testosterone, and estradiol.
• Assessed urinary 17-ketosteroids (17-KS).

Main Results:

• Markedly different adenylate kinase (ADK) activities were observed between HC and CC groups (P < 0.01).
• Significant differences in Na(+)-K(+)-ATPase activities were found between HC and CC groups (P < 0.05).
• The energy charge in the HC group was 16.1% higher than in the CC group.
• Serum levels of T3, T4, and progesterone were significantly higher in the HC group compared to the CC group (P < 0.01).
• No significant differences were found in estradiol (E2) or 17-KS levels between the groups.

Conclusions:

• The distinct physiological profiles suggest that cold-constitution (CC) and heat-constitution (HC) in Wistar rats are associated with fundamental differences in basic metabolism and hormonal regulation.
• These findings provide a biochemical basis for understanding constitutional typologies and their implications for physiological function.