Related Experiment Video
Updated: Aug 9, 2026

FISH for Pre-implantation Genetic Diagnosis
Published on: February 23, 2011
Multicolor banding detects a complex three chromosome, seven breakpoint unbalanced rearrangement in an ICSI-derived
Mary J Seller1, Susan Bint, Fred Kavalier
1Department of Medical and Molecular Genetics, King's College London School of Medicine, Guy's Hospital, London, UK. mary.seller@gentics.kcl.ac.uk
Abstract:
We describe a fetus from an intracytoplasmic sperm injection (ICSI) pregnancy with severe facial clefts, receding jaw, preauricular skin tags, postaxial hexadactyly, bi-lobed right lung, supernumerary cranial bone, and dilated lateral ventricles of the brain. Using a combination of G-banding, fluorescence in situ hybridization (FISH), whole chromosome paints (WCPs), subtelomere probes, and multicolor banding (MCB), the karyotype was found to include a de novo unbalanced highly complex chromosome rearrangement (hCCR) involving chromosomes 3, 12, and 15 with seven breakpoints, and including monosomy for two separate regions of chromosome 12.
More Related Videos
10:14Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice
Published on: January 11, 2017
12:32Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
Published on: September 7, 2021
Related Concept Videos
Karyotyping
Meiosis I
Nondisjunction
Nondisjunction
Meiosis vs. Mitosis
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...