Related Experiment Video
Updated: Aug 9, 2026

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
Published on: August 20, 2012
Fluorescent ratiometry of tetrahomodioxacalix[4]arene pyrenylamides upon cation complexation
Jung Kyu Choi1, Areum Lee, Seyoung Kim
1Department of Chemistry, Institute of Nanosensor & Biotechnology, Dankook University, Seoul 140-714, Korea.
Abstract:
[structure: see text] C-1,2-alternate tetrahomodioxacalix[4]arene pyreneamides were synthesized. Pb(2+) coordination gave a quenched monomer and excimer fluorescence emission, while upon Ca(2+) ion binding, the receptor provides an enhanced excimer and declined monomer emission with ratiometric response. The excimer emission spectra changes are rationalized by frontier molecular orbitals that the effective Py-Py interaction induces emission intensity increases upon Ca(2+) ion complexation, whereas there is no such interaction observed upon Pb(2+) binding.
Related Concept Videos
Photoluminescence: Applications
Variables Affecting Phosphorescence and Fluorescence
Aryldiazonium Salts to Azo Dyes: Diazo Coupling
Complexometric Titration: Overview

