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Updated: Aug 9, 2026
Natural Product Discovery with LC-MS/MS Diagnostic Fragmentation Filtering: Application for Microcystin Analysis
Published on: May 31, 2019
[Production purification and characterization of polyantibodies against microcystin-LR]
Xiao-Lian Zhao1, Wei-Rong Sun, Yuan Liu
1School of Food Science of Southern Yangtze University, Wuxi 214036, China.
Objective:
Production and characterization the polyantibodies against MCYST-LR.
Methods:
microcystin-LR (MC-LR) was conjugated to KLH, then immune the rabbit by the routine method. Purify the antiserum by centrifugal and saturated sulfuric ammonium precipitated methods. Count the affinity constant and measure the titers, IC50 Value and the sensitivity.
Results:
The titers of the antiserum is over 25600. IC50 value is about from 0.1 ng/ml to 1 ng/ml. The across-reactivity ratio to MC-LW and MC-LR is about from 0.1% to 20%.
