Related Experiment Video
Updated: Aug 9, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
Published on: May 5, 2016
Potential of two-photon-excited fluorescence for distributed fiber sensing
Ivan S Ruddock1, Thomas P J Han
1Department of Physics, University of Strathclyde, Glasgow G4 0NG, UK. i.s.ruddock@strath.ac.uk
Abstract:
The design and operating parameters of a new class of continuously distributed optical fiber sensor are described. By use of counterpropagating pulses, two-photon excitation of fluorescence from ions doped into the fiber enables any position to be monitored. By this means temperature and also strain may be sensed with high spatial and temporal resolution. As the doped fiber is transparent for single-photon absorption at the wavelength of the light pulses, attenuation does not set an upper limit to its length.
Related Concept Videos
Fluorescence and Phosphorescence: Instrumentation
Photoluminescence: Fluorescence and Phosphorescence
A pair of electrons in a...
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

