Preparation and characterization of EP-liposomes and Span 40-niosomes

Clotrimazole, an imidazole derivative antifungal agent, has been widely used for the treatment of mycotic infections of the genitourinary tract. In order to develop alternative formulations for the vaginal administration of clotrimazole which would provide sustained and controlled release of the appropriate drug for local vaginal therapy, liposomes/niosomes were evaluated as delivery vehicles. To optimize the preparation of the liposomes/niosomes with regard to size and entrapment efficiency, multilamellar liposomes/ niosomes containing the drug were prepared by a lipid hydration method. The liposomes/niosomes thus prepared were evaluated for their stability as drug-loaded liposomes/niosomes in simulated vaginal fluid at 37 +/- 1 degrees C. The two vesicle systems were also evaluated with regard to rat and rabbit vaginal irritation.